Regulatory T Cell Staining Kit

Distributor quantity price mouse regulatory t cell staining kit 3 kit. Immunoblotting with fjk 16s antibody has mapped the epitope to amino acids 75 125 of the mouse foxp3 protein. This human regulatory t cell staining kit contains all of the buffers and monoclonal antibodies for cd4 cd25 and foxp3 necessary to successfully stain and identify regulatory t cells from human peripheral blood cells the rpa t4 monoclonal antibody reacts with human cd4 a 59 kda glycoprotein found.

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One kit is sufficient for staining 25 samples.

Regulatory t cell staining kit.

Foxp3 a 49 55 kda protein is a member of the forkhead winged helix family of. Mouse regulatory t cell staining kit 2 kit from thermo fisher scientific. In our experience the human regulatory t cell staining kit from ebioscience is a reliable and straightforward tool for detection of cd4 cd25 treg cells expressing foxp3. This human regulatory t cell staining kit contains all of the buffers and monoclonal antibodies for cd4 cd25 and foxp3 necessary to successfully stain and identify regulatory t cells from human peripheral blood cells the rpa t4 monoclonal antibody reacts with human cd4 a 59 kda glycoprotein found on the surface of the majority of thymocytes a.

The following protocol allows the simultaneous analysis of cell surface molecules and intracellular antigens including nuclear antigens. Immunoblotting with fjk 16s antibody has mapped the epitope to amino acids 75 125 of the mouse foxp3 protein. Not for use in diagnostic procedures. 88 8115 ebioscience mouse regulatory t cell staining kit 3 fjk 16s for research use only.

Constitutive high expression of foxp3 mrna has been shown in cd4 cd25 regulatory t cells treg cells and ectopic expression of foxp3 in cd4 cd25 cells imparts a treg phenotype in these cells. This human regulatory t cell whole blood staining kit contains the buffers and monoclonal antibody necessary to successfully stain and identify foxp3 cells in whole blood samples. The pch101 monoclonal antibody reacts with the amino terminus of human foxp3 also known as forkhead box p3 scurfin and jm2. Foxp3 staining protocol for mouse tissues introduction.

Foxp3 is a 49 55 kda protein and a. Cross reactivity of this antibody to other proteins has not been determined. Constitutive high expression of foxp3 mrna has been shown in cd4 cd25 regulatory t cells treg cells and ectopic expression of foxp3 in cd4 cd25 cells imparts a treg phenotype in these cells. Expanded effectors or tils.

The treg detection kit cd4 cd25 foxp3 includes all reagents necessary for cell surface staining of cd4 and cd25 and intracellular staining of foxp3 with a special foxp3 staining buffer set. Ebioscience 88 8115 40 product information. Description the fjk 16s antibody reacts with mouse rat foxp3 also known as forkhead box p3 scurfin and jm2. The kit also includes an optimized protocol.

The protocol recommends using 1 x 10 6 pbmcs but it can be adjusted for staining other cells e g.

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